General Principles of Cell Communication

Cell communication is the complicated system by which cells control and regulate cell functions, by which development, immune response, tissue repair and homeostasis are based upon. Cells in the human body exists as a tissue, if isolated, it will not

General Principles of Cell Communication

Cell communication is the complicated system by which cells control and regulate cell functions, by which development, immune response, tissue repair and homeostasis are based upon. Cells in the human body exists as a tissue, if isolated, it will not

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RNA Interference – Overview

Pre-requisites: Understanding of RNA strucutre and translation When scientists injected extra copies of a pigment-enhancing gene to petunias, rather than observing the flower’s colour becoming more intense, they observed flowers with variegated petals with sections containing no colour. When they

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RNA Interference – Overview

Pre-requisites: Understanding of RNA strucutre and translation When scientists injected extra copies of a pigment-enhancing gene to petunias, rather than observing the flower’s colour becoming more intense, they observed flowers with variegated petals with sections containing no colour. When they

Analysing Gene Function

The function of a gene can be determined in two ways: by experimentation, removing, impairing, adding or over-expressing a gene to an organism and observe the altered phenotype, from this a mechanism is hypothesised and scrutinised until the function is

Analysing Gene Function

The function of a gene can be determined in two ways: by experimentation, removing, impairing, adding or over-expressing a gene to an organism and observe the altered phenotype, from this a mechanism is hypothesised and scrutinised until the function is

Gene Cloning and PCR

The definition of gene cloning is the transfer of a DNA molecule from a source to a host organism, and through recombination is incorporated into a vector (the genome, or a plasmid) of the host such that its progeny will

Gene Cloning and PCR

The definition of gene cloning is the transfer of a DNA molecule from a source to a host organism, and through recombination is incorporated into a vector (the genome, or a plasmid) of the host such that its progeny will

Applications of PCR

Amplify DNA Identify the presence and concentration of a DNA segment in a sample – If it is present, when ran on a gel, there should be a band which corresponds to the topology and size of the DNA fragment

Applications of PCR

Amplify DNA Identify the presence and concentration of a DNA segment in a sample – If it is present, when ran on a gel, there should be a band which corresponds to the topology and size of the DNA fragment

Expression of Foreign Genes

Prerequisites – Understand transcription and translation, gene and DNA cloning.   To obtain proteins from its native sources are not always economical because it normally requires a large amount of starting material, and the proteins may be hard to purify

Expression of Foreign Genes

Prerequisites – Understand transcription and translation, gene and DNA cloning.   To obtain proteins from its native sources are not always economical because it normally requires a large amount of starting material, and the proteins may be hard to purify

Walkthrough – Phage Transduction

Prerequisites – A basic understanding of the structure of DNA and the function of a gene Introduction Phage transduction is the transfer of DNA fragments from a donor strain to a different recipient strain via a bacteriophage. There are two

Walkthrough – Phage Transduction

Prerequisites – A basic understanding of the structure of DNA and the function of a gene Introduction Phage transduction is the transfer of DNA fragments from a donor strain to a different recipient strain via a bacteriophage. There are two